Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Egg chamber
NA
NA

Attributes by original data submitter

Sample

source_name
stage 10 egg chambers from OrR
strain
OrR
chip antibody
IgG (made in-house)
developmental stage
stage 10
tissue
egg chamber

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Egg chambers were dounced and sonicated using a Biorupto 300 (Diagenode) Libraries were constrcted using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
17095851
Reads aligned (%)
40.1
Duplicates removed (%)
46.0
Number of peaks
493 (qval < 1E-05)

dm3

Number of total reads
17095851
Reads aligned (%)
40.3
Duplicates removed (%)
43.3
Number of peaks
505 (qval < 1E-05)

Base call quality data from DBCLS SRA