Antigen

Antigen Class

TFs and others

Antigen

CDC45L

Cell type

Cell type Class

Adult

Cell type

Egg chamber

NA

NA

Sample

source_name

stage 10 egg chambers from OrR

strain

OrR

chip antibody

Cdc45 (made in-house)

developmental stage

stage 10

tissue

egg chamber

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Egg chambers were dounced and sonicated using a Biorupto 300 (Diagenode) Libraries were constrcted using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

10858467

Reads aligned (%)

6.6

Duplicates removed (%)

52.2

Number of peaks

173 (qval < 1E-05)

dm3

Number of total reads

10858467

Reads aligned (%)

6.8

Duplicates removed (%)

52.0

Number of peaks

135 (qval < 1E-05)