SRX7064292
GSM4142031: KBM7 H3K27ac; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
KBM-7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
KBM7 chronic myeloid leukemia cell line
cell line
KBM7
cell type
chronic myeloid leukemia cell line
chip antibody
H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard DNA extraction from KBM7 cells using cell lysis. Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 60% amplitude) on ice. Library construction using Rubicon ThruPLEX kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
58990025
Reads aligned (%)
97.7
Duplicates removed (%)
10.1
Number of peaks
41004 (qval < 1E-05)

hg19

Number of total reads
58990025
Reads aligned (%)
97.4
Duplicates removed (%)
10.5
Number of peaks
41060 (qval < 1E-05)

Base call quality data from DBCLS SRA