GSM4141516: TH1 CD4+ T cells replicate 1; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
memory CD4+ T cells from pooled mice splenic extracts
cell type
memory CD4+ T cells
strain
T-bet ZSGREEN
Sex
pooled male and female
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Single cells were extracted from suspensions of spleen from 4 male and 2 female individual mice that were pooled for sorting TH1 and TFH cells or one individual female litter-mate mice for naive CD4 T cells Cells were sorted by flow cytometry (using a FACSAria III, BD Biosciences) and gated for dump-CD4+CD44+gp66+ expression and either Ly6C+ PSGL-1+ (Th1) Ly6C-PSGL-1- (TFH) or dump- CD4+ CD44- CD62L+ for naive CD4 T cells. The sorted cell suspension from each sample were prepared according to Omni ATAC protocol that was previously published In brief, DNA was isolated, tagmented with a transposase, amplified by PCR and purified