GSM4141466: HEK293 Phase Inhibition 5min Pol2 ChIP-seq.rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293 cells
ip method or chip antibody
Pol II(8WG16):BioLegend No. 664911
genotype
Wild type
treatment
Treated with 1.5% (v/v) 1,6-hexanediol (Sigma, 240117) for 5min
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
5 million cells were used for all ChIP reactions. Crosslinked cells were resuspend in ice-cold lysis buffer (50 mM HEPES, pH=7.9, 5 mM MgCl2, 0.2% Triton X-100, 20% glycerol, 300 mM NaCl) and incubated on ice for 10 min before centrifuged at 500 g for 5 m