GSM4138742: NDN ctrl 08 [ATAC-seq]; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
Neutrophils
MeSH Description
Immature neutrophils.
Attributes by original data submitter
Sample
source_name
Human whole blood
diagnosis
Healthy (control)
cell type
NDN
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Peripheral blood was collected by heparinized tubes and the PBMC fraction was obtained using Ficoll/Hypaque gradient. LDGs were sorted by flow cytometry using CD10-APC,CD14-FITC and CD15-PE antibodies. ATAC-seq was performed according to a published protocol (Buenrostro et al., 2013) with minor modification. 100,000 cells performed in quadruplicate for each sample were treated with the Tn5 transposase (Illumina) for 30 min at 37°C and PCR-purified (Qiagen) to generate library fragments. ATAC seq data were generated on Illumina HiSeq 2500 instruments according to the manufacturer's instructions.