Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

mouse G1E-ER4 cells

tissue

Mouse G1E-ER4 cells

chip antibody

H3K27ac, Abcam, ab4729, GR132150-4

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq analysis using the Illumina NextSeq500, 5 ng of ChIP DNA was processed for library generation using the NEBNext ChIP-seq Library Prep Master Mix following the manufacturer's protocol (New England Biolabs). Raw reads that aligned to exactly one location in the reference human genome (hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

9881423

Reads aligned (%)

97.7

Duplicates removed (%)

9.4

Number of peaks

18272 (qval < 1E-05)

mm9

Number of total reads

9881423

Reads aligned (%)

97.4

Duplicates removed (%)

9.5

Number of peaks

18260 (qval < 1E-05)