Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4

tissue

Mouse G1E-ER4 cells

time point

24hr

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

For ATAC-seq analysis using the Illumina NextSeq500, ~50,000 cells were processed for tagmentation followed by DNA isolation and library generation. Raw reads that aligned to exactly one location in the reference mouse genome (mm10) were retained for downstream data analysis.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

8065611

Reads aligned (%)

86.8

Duplicates removed (%)

16.0

Number of peaks

2832 (qval < 1E-05)

mm9

Number of total reads

8065611

Reads aligned (%)

86.6

Duplicates removed (%)

16.1

Number of peaks

2829 (qval < 1E-05)