GSM4128444: CRL7250 WT RING1B ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RNF2
Cell type
Cell type Class
Others
Cell type
Fibroblasts
NA
NA
Attributes by original data submitter
Sample
source_name
CRL7250 (FIbroblast)
cell type
FIbroblast
cell line
CRL7250
chip antibody
CST (D22F2)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the NEBNext® Ultra™ II kit (New England BioLabs )