Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Splenic B cells, Input
genotype
--
cell isolation
Splenic B cells
condition
Ex-vivo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Genome Analyzer Iix following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
7047409
Reads aligned (%)
89.1
Duplicates removed (%)
17.1
Number of peaks
1371 (qval < 1E-05)

mm9

Number of total reads
7047409
Reads aligned (%)
88.8
Duplicates removed (%)
17.5
Number of peaks
1455 (qval < 1E-05)

Base call quality data from DBCLS SRA