Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Embryo
Cell type
2-cell stage
NA
NA

Attributes by original data submitter

Sample

source_name
2-cell embryo
cell type
2-cell embryo
pooled numbers
118
strain
C57BL/6
chip antibody
H3K9me3 (Pool of Abcam#ab8898, Lot GR153112-1 and Abcam#ab176916)
genotype
WT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Qiagene Ultra low input library preperation kit (180492) according to manufacturers instructions and quantified using KAPA library quantification kit from Roche (07960298001)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
70892297
Reads aligned (%)
81.9
Duplicates removed (%)
49.1
Number of peaks
933 (qval < 1E-05)

mm9

Number of total reads
70892297
Reads aligned (%)
81.7
Duplicates removed (%)
49.2
Number of peaks
938 (qval < 1E-05)

Base call quality data from DBCLS SRA