Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA

Attributes by original data submitter

Sample

source_name
WT fibroblasts_BRD4_ChIPSeq
cell type source
age-matched control wild-type individual 2 (TRS-WT)
cell type
WT dermal fibroblasts transformed by retroviral introduction of TERT, HRAS and SV40 T antigens (TRS-WT1)
chip antibody
BRD4
chip antibody vendor
Bethyl labs

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Library construction was performed following Illumina TruSeq 2.0 protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
37809738
Reads aligned (%)
132.9
Duplicates removed (%)
30.2
Number of peaks
452 (qval < 1E-05)

hg38

Number of total reads
37809738
Reads aligned (%)
135.1
Duplicates removed (%)
29.9
Number of peaks
953 (qval < 1E-05)

Base call quality data from DBCLS SRA