Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

Bone Marrow

tissue

Bone Marrow

cell type

Monocyte

infection

Steady State

strain

C57BL/6

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

20000 sorted monocytes were washed with cold PBS and resuspended in cold lysis buffer (10 mM Tris·Cl, pH 7.4, 10 mM NaCl,3 mM MgCl2, 0.1% (v/v) Igepal CA-630). Nuclei were subject to transposition reaction in 1x TD Buffer containing 2.5 μl transposase Nextera enzyme and DNA was purified. Purified DNA was amplified by PCR with 10-12 cycles. Amplified libraries were purified and quality controlled.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

45264567

Reads aligned (%)

68.6

Duplicates removed (%)

53.0

Number of peaks

31500 (qval < 1E-05)

mm9

Number of total reads

45264567

Reads aligned (%)

68.5

Duplicates removed (%)

53.0

Number of peaks

31452 (qval < 1E-05)