GSM1500010: Mock DNase-Seq 60 min Cort Pulsed; Mus musculus; DNase-Hypersensitivity
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Breast
Cell type
3134
Tissue
Mammary
Lineage
cellLine
Description
Mammary
Attributes by original data submitter
Sample
source_name
3134 cells
cell line
3134
cell type
mouse mammary cells
treatment
600 nM Cort for 20 min + 40 min in hormone-free medium
Sequenced DNA Library
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
Lysates were clarified from sonicated nuclei. DHS-seq: Nuclei were isolated and subjected to a limited DNase I digestion. Small DNA fragments where DNase I cut twice were isolated and prepared for sequencing according to the manufacturer (Illumina).