Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Breast

Cell type

3134

Tissue

Mammary

Lineage

cellLine

Description

Mammary

Sample

source_name

3134 cells

cell line

3134

cell type

mouse mammary cells

treatment

none (untreated cells)

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

Lysates were clarified from sonicated nuclei. DHS-seq: Nuclei were isolated and subjected to a limited DNase I digestion. Small DNA fragments where DNase I cut twice were isolated and prepared for sequencing according to the manufacturer (Illumina).

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

23803770

Reads aligned (%)

97.3

Duplicates removed (%)

31.3

Number of peaks

54986 (qval < 1E-05)

mm9

Number of total reads

23803770

Reads aligned (%)

97.1

Duplicates removed (%)

31.3

Number of peaks

55045 (qval < 1E-05)