GSM1499996: Pol II ChIP-Seq Mock 60 min Cort Pulsed 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Breast
Cell type
3134
Tissue
Mammary
Lineage
cellLine
Description
Mammary
Attributes by original data submitter
Sample
source_name
Pol II ChIP-Seq Mock 60 min Cort Pulsed
cell line
3134
cell type
mouse mammary cells
treated with
600 nM Cort for 20 min + 40 min in hormone-free medium
chip antibody
RNA polymerase II phosphoS5 antibody
chip antibody vendor
Abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes, Pol II-DNA complexes or CTCF-DNA complexes were isolated with antibody. NEB Chip-Seq Prep Kit