Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nr3c1

Cell type

Cell type Class
Breast
Cell type
3134
Tissue
Mammary
Lineage
cellLine
Description
Mammary

Attributes by original data submitter

Sample

source_name
GR ChIP-Seq 20 min Cort
cell line
3134
cell type
mouse mammary cells
treated with
600 nM Cort for 20 min
chip antibody
GR Antibody
chip antibody vendor
Affinity BioReagents; Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes, Pol II-DNA complexes or CTCF-DNA complexes were isolated with antibody. NEB Chip-Seq Prep Kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
25584480
Reads aligned (%)
82.1
Duplicates removed (%)
39.0
Number of peaks
7309 (qval < 1E-05)

mm9

Number of total reads
25584480
Reads aligned (%)
81.9
Duplicates removed (%)
39.1
Number of peaks
7316 (qval < 1E-05)

Base call quality data from DBCLS SRA