Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Others

Cell type

Palate

MeSH Description

The structure that forms the roof of the mouth. It consists of the anterior hard palate (PALATE, HARD) and the posterior soft palate (PALATE, SOFT).

Sample

source_name

Anterior Palate

strain

Shox2HA-DsRed

antibody

anti-H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The anterior and posterior palate tissues were dissected out under a fluorescence dissecting scope from Shox2HA-DsRed embryos. Pooled palatal tissues were subjected to ChIP using anti-HA (Abcam, ab9110) or anti-H3K27ac antibodies (Abcam, ab4729) following the manufacturer's protocol. Three independent ChIPs were conducted for library generation for each group. The libraries were then sequenced on the Illumina HiSeq 2000 platform using 50-bp single-end configuration. HA-enriched or H3K27ac-enriched genomic regions of the Shox2-expressing anterior palate and the posterior palate were analyzed and intersected comprehensively. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

25302880

Reads aligned (%)

96.0

Duplicates removed (%)

3.9

Number of peaks

7117 (qval < 1E-05)

mm9

Number of total reads

25302880

Reads aligned (%)

95.9

Duplicates removed (%)

4.0

Number of peaks

7116 (qval < 1E-05)