Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

CTCF_Control

cell line

HeLa

treatment

None

chip antibody

CTCF

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed with an anti-CTCF antibody (Active Motif, 61311) as described [PMID: 25646466, 26704968] for two biological replicates. ChIP samples were prepared for next-generation sequencing using a NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs).

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

42185806

Reads aligned (%)

95.7

Duplicates removed (%)

11.3

Number of peaks

42425 (qval < 1E-05)

hg19

Number of total reads

42185806

Reads aligned (%)

95.1

Duplicates removed (%)

12.4

Number of peaks

41874 (qval < 1E-05)