Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Embryo

Cell type

Cloaca

MeSH Description

A dilated cavity extended caudally from the hindgut. In adult birds, reptiles, amphibians, and many fishes but few mammals, cloaca is a common chamber into which the digestive, urinary and reproductive tracts discharge their contents. In most mammals, cloaca gives rise to LARGE INTESTINE; URINARY BLADDER; and GENITALIA.

Sample

source_name

cloaca region

tissue

Cloaca

antibody

H3K27ac

genotype

wild type

developmental stage

E10.5

strain

CBA / C57BL/6 mix

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Micro-dissected GT (35 to 40) or CR (70) were crosslinked in 1% formaldehyde/PBS for 20 min and stored at -80oC until further processing. Chromatin was sheared using a water-bath sonicator (Covaris E220 evolution ultra-sonicator). Immunoprecipitation was done using the following antibodies, anti-CTCF (Active Motif, 61311), anti- H3K27ac (Abcam, ab4729), and H3K27me3 (Merck Millipore, 07-449) Libraries were prepared using the TruSeq protocol, and sequenced on the Illumina HiSeq system (100bp single-end reads) according to manufactures instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

10639067

Reads aligned (%)

85.0

Duplicates removed (%)

30.9

Number of peaks

1908 (qval < 1E-05)

mm9

Number of total reads

10639067

Reads aligned (%)

84.8

Duplicates removed (%)

31.0

Number of peaks

1868 (qval < 1E-05)