Antigen

Antigen Class

TFs and others

Antigen

EZH2

Cell type

Cell type Class

Prostate

Cell type

42D

NA

NA

Sample

source_name

Human prostate cancer cell line

cell line

enzalutamide (ENZ)-resistant tNEPC 42D

tumour type

Prostate neuroendocrine

chip antibody

EZH2 (Active Motif, #39933)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP sequencing libraries (100 ng DNA per sample) were constructed using the KAPA HyperPrep Kit (Roche, #KK8504) with Illumina TruSeq indexes. Sequencing was performed on an Illumina NextSeq 500 to generate 75-bp single-end reads.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

55448319

Reads aligned (%)

85.5

Duplicates removed (%)

10.5

Number of peaks

12735 (qval < 1E-05)

hg19

Number of total reads

55448319

Reads aligned (%)

84.7

Duplicates removed (%)

12.1

Number of peaks

12434 (qval < 1E-05)