Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Neural
Cell type
SK-N-SH
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
neuroblastoma-derived cell line
growth media
RPMI 1640, 10% FBS, 1% Penicillin/Streptomycin, 2mM L-Glutamine
cell line
SKNSH
mycn status
non-amp
chip antibody
C-MYC (Santa Cruz N262, #sc-764)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, flash frozen, and sent to Active Motif. According to Active Motif protocol, cells were lysed, sonicated, and then DNA-protein complexes were isolated with antibodies. Samples were reverse-crosslinked and DNA was purified. Illumina libraries were prepared by Active Motif using standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After 15 cycles of PCR amplification, the resulting DNA libraries were quantified and sequenced on Illumina NextSeq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
34824400
Reads aligned (%)
26.0
Duplicates removed (%)
72.7
Number of peaks
6395 (qval < 1E-05)

hg38

Number of total reads
34824400
Reads aligned (%)
27.5
Duplicates removed (%)
71.4
Number of peaks
6521 (qval < 1E-05)

Base call quality data from DBCLS SRA