Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Input_DNA_JMJD3-floxed

strain

C57BL/6

tissue

Primary liver cells

treatment

JMJD3-floxed (liver-specific JMJD3 knockdown by AAV-TBG-Cre)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Finely minced liver tissue was incubated in PBS containing 1% formaldehyde for 10 min, and then, glycine was added.The samples were resuspended in hypotonic buffer and lysed by homogenization. Nuclei were pelleted and sonicated. The chromatin sample was precleared and immunoprecipitated by binding to Protein G agarose with the H3K27-me3 (Abcam) antibody, the beads were washed, chromatin was eluted, and DNA was isolated. ChIP assays were performaed using H3K27-me3 antibody (Ab6002) . Two sets of input and immunoprecipitated samples from 2 mices were used respectively. Libraries were prepared accoding to standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

26699480

Reads aligned (%)

95.9

Duplicates removed (%)

65.0

Number of peaks

369 (qval < 1E-05)

mm9

Number of total reads

26699480

Reads aligned (%)

95.3

Duplicates removed (%)

65.0

Number of peaks

374 (qval < 1E-05)