Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Erythroblasts

MeSH Description

Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Sample

source_name

primitive erythroblasts

embryonic day

E10.5

tissue

Peripheral Blood

genotype

setd8-null

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Primitive erythroblasts were removed from peripheral blood by dissection in PBS supplemented with glucose and BSA Briefly, nuclei were collected from Setd8 null and control erythroblats following cell lysis and subjected to tagmentation using transpoase (Ilumina Nextera FC121-1030). Samples were then amplified x 5 PCR cycles [980C/45s + 5 x (980C/15s + 630C/30s + 720C/30s) + 720C/1min] using KAPA HiFi HotStart ReadyMix PCR Kit (KK2601/KK2602) and cleaned with Qiagen MiniElute column. The library was then amplified with PCR [980C/45s + Y x (980C/15s + 630C/30s + 720C/30s) + 720C/1min], with the number of cycles (Y) determined through via a qPCR library amplification test. The resulting PCR producte was cleaned using Agencourt AMPure XP magnetic beads (Beckman Coulter A63880)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

31480210

Reads aligned (%)

67.1

Duplicates removed (%)

96.2

Number of peaks

1244 (qval < 1E-05)

mm9

Number of total reads

31480210

Reads aligned (%)

67.0

Duplicates removed (%)

96.3

Number of peaks

1225 (qval < 1E-05)