GSM4099036: K562-BRD3-DMSO-noIFNG; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD3
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
erythroleukaemia
cell line
K562
inhibitor treatment
DMSO
stimulation
noIFNγ
stimulation protocol
None
antibody
BRD3(Bethyl; A302-368A)
replicate
1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20 million cells were crosslinked for 15 mins with 1% formaldehyde. Crosslinked material was sonicated to ~200–1000 bp using the Covaris Ultrasonicator e220. Sonicated material was incubated overnight with each antibody, then incubated for 3 h with Protein A magnetic beads. Beads were washed with low and high salt wash buffers, LiCl buffer and TE, before being eluted and decrosslinked overnight. DNA was purified using Qiagen Minelute columns. All ChIP antibodies were used at ~10μg per IP Sequencing libraries were prepared from eluted DNA using Rubicon ThruPLEX DNA- seq kit. Libraries were size selected between 200–500 bps