Genomic DNA was isolated using the Qiagen DNeasy Blood and Tissue kit (Cat No./ID: 69504). Purified gDNA was fragmented using a Covaris S2 Sonicator. Libraries were prepared with 10ug gDNA using the Ovation® Ultralow Methyl Seq DR Multiplex System 1-8, 9-16 (NuGen 0335 and 0336). Libraries were pooled and sequenced at 100PE on a HiSeq 2000 in standard run mode to a read depth of >180M mapped reads at the Harvard FAS core.