Antigen

Antigen Class

TFs and others

Antigen

ZMYND8

Cell type

Cell type Class

Neural

Cell type

Glioblastoma

MeSH Description

A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.

Sample

source_name

pediatric glioblastomas from the cerebral cortex

chip antibody

RACK7(Bethyl)

genotype

wildtype

tumor

glioblastomas

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were lysed with lysis buffer and the whole cell lysate was sonicated with a Biorupter sonicator (Diagenode). The RACK7-DNA complexes were isolated with RACK7 antibody. The sequencing libraries for ChIP-seq were prepared from 2-5ng of DNA using the KAPA Hyper Prep Kits (KAPA, KK8504) according to the manufacturer's instructions. Completed ChIP-seq libraries were qualified by Bioanalyzer 2100 (Agilent).

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

20932705

Reads aligned (%)

80.7

Duplicates removed (%)

26.7

Number of peaks

489 (qval < 1E-05)

hg19

Number of total reads

20932705

Reads aligned (%)

79.7

Duplicates removed (%)

27.0

Number of peaks

228 (qval < 1E-05)