Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
697
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
pre-B leukemic 697 cells
cell line
pre-B leukemic cell line 697
treatment
parental 697
chip antibody
anti-RUNX1 (ab23980, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNAs were extrated using Quick-RNA Miniprep kit (Zymo Research) . Chromatin lysates were prepared by sonication and protein-DNA complexes were isolated with indicated antibodies followed by purification with PCR purification kit (Qiagen). Multiplexing libraries were prepared for sequencing using Illumina standard protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
36956521
Reads aligned (%)
88.0
Duplicates removed (%)
10.6
Number of peaks
65402 (qval < 1E-05)

hg38

Number of total reads
36956521
Reads aligned (%)
89.2
Duplicates removed (%)
9.8
Number of peaks
65572 (qval < 1E-05)

Base call quality data from DBCLS SRA