Antigen

Antigen Class

TFs and others

Antigen

Mef2d

Cell type

Cell type Class

Neural

Cell type

Cerebellum

MeSH Description

The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.

Sample

source_name

Cerebellum

genotype

MEF2D Conditional Knockout

Sex

female

strain

C57BL/6

chip antibody

MEF2D (Greenberg Lab)

tissue

Cerebellum

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted from the cerebella of P22 mice using Trizol (Invitrogen) according to the manufacturer's instructions. ChIP lysates clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

22918993

Reads aligned (%)

98.1

Duplicates removed (%)

23.7

Number of peaks

373 (qval < 1E-05)

mm9

Number of total reads

22918993

Reads aligned (%)

97.8

Duplicates removed (%)

23.8

Number of peaks

426 (qval < 1E-05)