Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

ChIP_Stag2KO_Input_4056_IGO

cell type

hematopoietic stem and progenitor cells (HSPCs)

genotype

Stag2 KO

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer's recommended protocol (New England Biolabs, Ipswich, MA, USA) for CTCF, Stag1, and Stag2 and Swift Accel-NGS 2S Plus (Swift) for Smc1a and Smc3.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

25468860

Reads aligned (%)

98.8

Duplicates removed (%)

24.0

Number of peaks

149 (qval < 1E-05)

mm9

Number of total reads

25468860

Reads aligned (%)

98.6

Duplicates removed (%)

24.7

Number of peaks

145 (qval < 1E-05)