Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Gonad

Cell type

Spermatogonia

MeSH Description

Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.

Sample

source_name

testis

tissue

testis

cell type

differentiated spermatogonia cells

development stage

6-8 days post-natal

chip antibody

H3K9me3 (Active Motif, 39161)

strain

C57BL/6

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

All sorted cells and H3K9me3 antibody incubated by magnetic beads were immunoprecipitated and DNA was finally isolated. The amount of DNA used for the ChIP: 6pg. The sequence libraries were generated using the KAPA Hyper Prep Kit for the Illumina platform (kk8504), following the manufacturer's instructions. Paired-end 150-bp sequencing was performed on Illumina HiSeq X10.

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

11323362

Reads aligned (%)

93.0

Duplicates removed (%)

30.8

Number of peaks

980 (qval < 1E-05)

mm9

Number of total reads

11323362

Reads aligned (%)

92.8

Duplicates removed (%)

30.8

Number of peaks

938 (qval < 1E-05)