GSM4083970: H3K4me3 0Dox ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
hESC H9 cell line
target
H3K4me3
cell line
H9
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed on CD34+ magnetic sorted samples (RUNX1-ETO and RUNX) and on non-adhered floating mixed progenitors (H3K4me3 and H3K27ac) after single crosslink. Antibodies were used against the following antigens (manufacturer, cat #): HA tag (Sigma, H6908), RUNX1 (ab23980, Abcam), H3K27ac (Abcam, ab4729), H3K4me3 (Merk, 07-473), H3K79me2 (Abcam, ab3594). For H3K79me2 ChIP, a concentration of at least 0.2% SDS on the chromatin suspension was used and immunoprecipitation was carried overnight. ChIP libraries for Illumina sequencing were prepared using the KAPA Hyper Prep Kit (Roche, KR0961), as detailed by the manufacturer. Quality control of the libraries was performed using a High Sensitivity DNA chip on an Agilent Technologies 2100 Bioanalyser instrument and libraries were quantified using the RT-qPCR-based method KAPA Library Quantification Kit for Illumina Sequencing Platforms, following the manufacturer's protocol.