Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
Thymus
NA
NA

Attributes by original data submitter

Sample

source_name
thymus
donor
117
Stage
late cortical thymic subpopulation
chip_target
H3K27me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei of sorted thymic subpopulations and histone-DNA complexes were isolated with antibody. Single-end sequencing 75bp was performed using Illumina NextSeq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
31381103
Reads aligned (%)
94.2
Duplicates removed (%)
9.4
Number of peaks
1245 (qval < 1E-05)

hg19

Number of total reads
31381103
Reads aligned (%)
93.0
Duplicates removed (%)
10.7
Number of peaks
1006 (qval < 1E-05)

Base call quality data from DBCLS SRA