Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Embryo

Cell type

Palatal shelves

NA

NA

Sample

source_name

palate cells

tissue

palate cells

developmental stage

E13.5

chip antibody

anti-FLAG

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with anti-FLAG antibody. Sequencing libraries were generated using Rubicon ThruPLEX DNA sequencing kit (Rubicon Genomics, Ann Arbor MI).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

44832697

Reads aligned (%)

90.6

Duplicates removed (%)

17.2

Number of peaks

10115 (qval < 1E-05)

mm9

Number of total reads

44832697

Reads aligned (%)

90.4

Duplicates removed (%)

17.2

Number of peaks

10053 (qval < 1E-05)