GSM1477435: E13.5 Female PGC KO H3K9me3; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Embryo
Cell type
Primordial germ cells
NA
NA
Attributes by original data submitter
Sample
source_name
primordial germ cells
cell type
SSEA1+ primordial germ cells
genotype
Setdb1 delta/- (KO)
Sex
female
antibody
H3K9me3 (Active Motif #39161)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Primordial germ cells (SSEA1+) and Somatic gonadal cells (SSEA-) were isolated from the gonads of E13.5 embryos and purified by FACS. Cells were sorted directly in nuclear isolation buffer containing RNAse inhibitor and protease inhibitor cocktail. For RNA-seq libraries, RNA was extracted using TriReagent, and dsCDNA was synthesized using Superscript III and Klenow fragment, respectively. For ChIP-seq libraries, DNA was extracted from immunoprecipitated DNA-protein complexes using phenol:chloroform:isoamyl alcohol 25:24:1. Adaptor ligation based library construction for paired-end Illumina sequencing.