SRX6854359
GSM4080858: WT K36me2 S2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me2

Cell type

Cell type Class
Pancreas
Cell type
Pancreatic cancer cells
NA
NA

Attributes by original data submitter

Sample

source_name
pancreatic cancer cells
genotype/variation
wildtype
cell type
Pancreatic cancer cells
chip antibody
H3K36me2 (Abcam, ab9049)
spike-in genome
Drosophila melanogaster genomic DNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Details in Methods section. Libraries were prepared according to the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) protocol.

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
61390968
Reads aligned (%)
98.0
Duplicates removed (%)
20.7
Number of peaks
281 (qval < 1E-05)

mm9

Number of total reads
61390968
Reads aligned (%)
97.8
Duplicates removed (%)
20.6
Number of peaks
349 (qval < 1E-05)

Base call quality data from DBCLS SRA