GSM4080150: Zfc3h1 KO 3 ESC RBP1 ChIPseq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Zfc3h1_KO_ESC_RBP1_ChIPseq
cell line
E14TG2a
cell type
Embryonic stem cells
genotype/variation
Zfc3h1-/-
chip/rip antibody
RPB1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ES cells were cross-linked by the addition of 1% formaldehyde for 10 minutes at RT before quenching with glycine. DNA was sheared to ~ 200 bp fragments by sonication using a Biorupter (Diagenode). ChIPs were carried out using 200 µg of chromatin and 2-3 µg of the indicated antibodies ChIPseq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB) using AmpureXP beads (Beckman) for size selection.