GSM4080142: Zfc3h1 KO 1 ESC H3K4me3 ChIPseq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Zfc3h1_KO_ESC_H3K4me3_ChIPseq
cell line
E14TG2a
cell type
Embryonic stem cells
genotype/variation
Zfc3h1-/-
chip/rip antibody
H3K4me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ES cells were cross-linked by the addition of 1% formaldehyde for 10 minutes at RT before quenching with glycine. DNA was sheared to ~ 200 bp fragments by sonication using a Biorupter (Diagenode). ChIPs were carried out using 200 µg of chromatin and 2-3 µg of the indicated antibodies ChIPseq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB) using AmpureXP beads (Beckman) for size selection.