NKp46+ ILC3 (CD3-CD19-CD127+CD90hiKLRG1-CCR6-NKp46+) and CCR6+ ILC3 (CD3-CD19-CD127+CD90hiKLRG1-CCR6+NKp46-) were sort purified from the small intestine lamina propria of pooled adult Maf+/+ Il7rCre and Maf fl/fl Il7rCre. For the ATAC-seq transitions DN ILC3s (CD3-CD19-NK1.1-/CD45loCD90.2hi/KLRG1-/NKp46-CCR6-) and NKp46+ ILC3s (CD3-CD19-NK1.1-/CD45loCD90.2hi/KLRG1-/NKp46+CCR6-) were sort purified from C57Bl/6 mice. Additionally, NKp46+ ILC3s (CD3-CD19-/CD45+CD127+ NKp46+RORgthi), NKp46+ RORgtlo (CD3-CD19-/CD45+CD127+ NKp46+RORgtlo), and ILC1s (CD3-CD19-/CD45+CD127+NKp46+RORgt-) were purified from Rorc(t) GFP/+ mice. Cells from 3-7 mice were pooled prior to sorting. The Omni-ATAC protocol was performed according to Corces et al (Nature Methods, 2017). Nuclei were subjected to Tn5 transposition (Illumina) and transposed DNA was purified using a Qiagen MinElute Kit prior to library amplification. Transposed DNA fragments were amplified with Nextera PCR primers for a total of 10-12 cycles using NEBNext High-Fidelity Polymerase (NEB).