Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NCOA3

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
Mammary Epthelial Cells
tissue
Normal Immortalized MCF10A Mammary Epithelial Cells
passage
10-15
antibody
CST 2126, Lot 4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Antibody-DNA complexes were isolated with antibody. Libraries were prepared according to ActiveMotif's Low Cell ChIP-seq Kit instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
69570848
Reads aligned (%)
162.7
Duplicates removed (%)
15.9
Number of peaks
834 (qval < 1E-05)

hg38

Number of total reads
69570848
Reads aligned (%)
164.5
Duplicates removed (%)
15.6
Number of peaks
1784 (qval < 1E-05)

Base call quality data from DBCLS SRA