Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

Spleen

strain

C57BL/6

tissue

Spleen

celltype

CD4+ Foxp3/RFP+ CD62L+ IL21/GFP- PD1- CXCR5-

genotype

Foxp3/RFP x IL21/GFP

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cell lysates were treated with Tn5 transposease and DNA was purified following the protocol outlined in Buenrostro et al. 2015 Curr. Protoc. Mol. Biol. 109:21.29.1-9 and Buenrostro et al 2013 Nat. Med. 10(12):1213-8. Libraries were generated following the following the protocol outlined in Buenrostro et al. 2015 Curr. Protoc. Mol. Biol. 109:21.29.1-9 and Buenrostro et al 2013 Nat. Med. 10(12):1213-8.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

43129259

Reads aligned (%)

82.8

Duplicates removed (%)

21.0

Number of peaks

11594 (qval < 1E-05)

mm9

Number of total reads

43129259

Reads aligned (%)

82.7

Duplicates removed (%)

21.0

Number of peaks

11548 (qval < 1E-05)