mut_CH12 stably expressed mutant CTCF (CTCF with the first two ZFs deleted), tagged with V5 Tag at the C-terminus) from vector (of pMy-MouseCTCFbiotag-T2A-mOrange)
treatment
none
chip antibody
RAD21 ABs Abcam (ab992)
mutation
homozygous deletion of ZF9-11 in CTCF gene
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitated DNA was purified with QIAquick columns (ZymaResearch). DNA concentration was assessed with a Qubit4 (ThermoFisher) and 5–10 ng was used to generate sequencing libraries using a TruSeq ChIP Sample Preparation Kit (Illumina, Inc., USA). TruSeq ChIP Sample Preparation Kit (Illumina, Inc., USA). RNA-seq libraries were prepared using either Ion Total RNA-seq v2 kit or TruSeq Stranded RNA LT Kit (Illumina).