Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Muscle

Cell type

JR-1

NA

NA

Sample

source_name

JR1

cell line

JR1

chip antibody

H3K27ac (Active Motif, 39133)

enrichment target

SNAI2

mechanistic classifications of chip protein target

Active enhancers

treatment

shRNA control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

[harvesting protocol] ChIP samples harvested after 1% formaldehyde fixation for 12 minutes, sonicated, processed and ChIP-enriched via standard protocols (Active Motif ChIP-IT HS kit). ChIP enriched DNA was treated with Proteinase K at 55 ˚C for 30 minutes followed by reversal of crosslinks by heating to 80 ˚C for 2 hours, and DNA purification by column (MiniElute PCR purification kit, Qiagen). DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. Standard Illumina barcodes were introduced during library preparation with the Illumina TruSeq ChIP Sample Prep Kit 48 Samples - Set A Cat# IP-202-1012

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

21238847

Reads aligned (%)

91.7

Duplicates removed (%)

25.0

Number of peaks

1511 (qval < 1E-05)

hg19

Number of total reads

21238847

Reads aligned (%)

91.2

Duplicates removed (%)

26.2

Number of peaks

1450 (qval < 1E-05)