Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Adipocyte
Cell type
SGBS
NA
NA

Attributes by original data submitter

Sample

source_name
adipocyte_RNAPII ChIP-seq TNF
cell type
SGBS adipocyte D10
treated with
10ng/ml TNFα for 90min
chip antibody
RNAPII
chip antibody vendor
Diagenode

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed according to standard protocol as described in (Siersbæk et al. 2012, MCB, 32: 3452-3463) ChIP-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg38

Number of total reads
15249945
Reads aligned (%)
98.3
Duplicates removed (%)
23.2
Number of peaks
21071 (qval < 1E-05)

hg19

Number of total reads
15249945
Reads aligned (%)
98.0
Duplicates removed (%)
23.2
Number of peaks
21042 (qval < 1E-05)

Base call quality data from DBCLS SRA