Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Adipocyte

Cell type

SGBS

NA

NA

Sample

source_name

adipocyte_RNAPII ChIP-seq Vehicle

cell type

SGBS adipocyte D10

treated with

vehicle for 90min

chip antibody

RNAPII

chip antibody vendor

Diagenode

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed according to standard protocol as described in (Siersbæk et al. 2012, MCB, 32: 3452-3463) ChIP-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

16141281

Reads aligned (%)

96.1

Duplicates removed (%)

14.6

Number of peaks

7697 (qval < 1E-05)

hg19

Number of total reads

16141281

Reads aligned (%)

95.6

Duplicates removed (%)

15.4

Number of peaks

7791 (qval < 1E-05)