sgRNA transduced OT-I cells from the B16-Ova melanoma-bearing mice
strain background
B16-Ova melanoma-bearing mice
cell type
sgRNA transduced OT-I cells
genotype/variation
BatfsKO
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were lysed in 50 μl ATAC-seq lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630 detergent) on ice for 10 min. Resulting nuclei were pelleted at 500g for 10 min at 4 °C. Supernatant was carefully removed with a pipette and discarded. The pellet was resuspended in 50 μl transposase reaction mix (25 μl 2 × TD buffer, 22.5 μl nuclease-free water, 2.5 μl transposase) and incubated for 30 min at 37 °C. After the reaction, the DNA was cleaned up using the Qiagen MinElute kit The barcoding reaction was run using the NEBNext HiFi kit on the basis of the manufacturer's instructions and amplified for five cycles