Antigen

Antigen Class

Histone

Antigen

H3K79me3

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Hela cells

cell type

Hela Cells

treatment

shAF9

chip antibody

Abcam Ab2621

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq were performed following the protocol: Using ChIP-seq technology to generate high-resolution profiles of histone modifications. O'Geen H, Echipare L, Farnham PJ. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. Library was contracted by the MD Anderson DNA sequencing Core facility following standard protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

67890340

Reads aligned (%)

95.8

Duplicates removed (%)

2.6

Number of peaks

1631 (qval < 1E-05)

hg19

Number of total reads

67890340

Reads aligned (%)

94.9

Duplicates removed (%)

4.3

Number of peaks

1498 (qval < 1E-05)