Antigen

Antigen Class

TFs and others

Antigen

CREB1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPSC derived neural cells

NA

NA

Sample

source_name

S133 phophorylation of CREB1 ChIP-Seq, iCell neurons, unstimulated, biorep 1, tech rep 1

tissue

cultured human iPSC-derived neurons at ~DIV16

time post-kcl stimulation

unstimulated

biological replicate

1

technical replicate

1

chip epitope

pCREB

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

nuclear isolation from homogenized cultures Nugen Ovation Ultralow Library System V1 using 2ng of starting DNA and performing 18 cycles of amplification

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

48361590

Reads aligned (%)

93.8

Duplicates removed (%)

67.2

Number of peaks

4081 (qval < 1E-05)

hg19

Number of total reads

48361590

Reads aligned (%)

93.3

Duplicates removed (%)

68.7

Number of peaks

4010 (qval < 1E-05)