For ATAC-seq (converted or not) nuclei were prepared from 50,000 cells for each transposition reaction. For ChIP-seq (converted or not), Lysates were clarified from sonicated nuclei and KLF4-bound chromatin was isolated with KLF4 antibody. For ATAC-seq and ChIP-seq, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. For bisulfite converted samples, a transposome was assembled with methylated adaptors for transposition reaction.