Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cells

genotype

TET2 knockout

chip antibody

anti-H3K4me1, Active Motif, 39297

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library preparation and sequencing procedures were carried out as described previously according to Illumina protocols with minor modifications (Illumina, San Diego).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

21527748

Reads aligned (%)

96.1

Duplicates removed (%)

13.0

Number of peaks

3003 (qval < 1E-05)

hg19

Number of total reads

21527748

Reads aligned (%)

95.6

Duplicates removed (%)

13.7

Number of peaks

3032 (qval < 1E-05)